Mass spectrometry compatible salt formation for ionic liquid sample preparation
AssigneeAgilent Technologies, Inc.
InventorGenevieve Van de Bittner
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Abstract
Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.
Claims20
A method for preparing an aqueous solution comprising metabolites and a mass spectrometry-compatible volatile salt or volatile compound, the method comprising:
- mixing a solution comprising metabolites and an ionic liquid, wherein the ionic liquid comprises a positively charged organic ion and an anion that can form a volatile salt or volatile compound, with a fluorous compound comprising a cation that can form a volatile salt or volatile compound, optionally in the presence of a fluorous solvent and/or other water-immiscible solvent or liquid, and thereby obtaining a two-layer mixture in which a first layer is not miscible with a second layer, the second layer is an aqueous solution comprising metabolites and the volatile salt or volatile compound and the first layer is a water-immiscible phase comprising the positively charged organic ion of the ionic liquid;
- and
- collecting the aqueous solution comprising metabolites and the volatile salt or volatile compound.
A method for manual or automatable ionic liquid workflow, the method comprising:
- 1) contacting a biological sample with one or more ionic liquids comprising one or more anions that can form a volatile salt or volatile compound, thereby obtaining a mixture comprising metabolites and the ionic liquid(s);
- 2) performing a metathesis reaction, and removing the cation of ionic liquid AB from the mixture comprising the metabolites by reacting the ionic liquid with a fluorous compound CD and producing a metabolite solution comprising a volatile salt or volatile compound;
- and
- 3) separating a water-immiscible phase from an aqueous phase, the aqueous phase comprising the metabolite solution.
A kit for obtaining a metabolite solution, the kit comprising one or more ionic liquids that comprises one or more anions that can form one or more volatile salts or volatile compounds and a fluorous compound comprising one or more cations that can form one or more volatile salts or volatile compounds;
- and wherein the kit further comprises one or more of the following:
- a phase separator material or column;
- a debris removal filtering device or column, a cell culture device that comprises a filter, a fluorous affinity column, a fluorous solvent, a water-immiscible solvent or liquid, a water-miscible solvent, a water-miscible solvent that is mixed with water, or any combination thereof.
Dependencies are inferred from the claim text.