Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims31
1-29. (canceled)
A system for analyzing analytes comprising a plurality of partitions, wherein a partition of said plurality of partitions comprises a first analyte, a second analyte, a cell feature binding group comprising a reporter molecule comprising a reporter barcode sequence associated with said cell feature binding group, and a support having coupled thereto a plurality of nucleic acid barcode molecules,
- wherein said second analyte is a different type of analyte than said first analyte,
- wherein said cell feature binding group is configured to couple to said first analyte, and
- wherein said plurality of nucleic acid barcode molecules comprise a common barcode sequence and a common capture sequence.
A system for analyzing analytes, comprising a plurality of partitions, wherein a partition of said plurality of partitions comprises a messenger ribonucleic acid (mRNA) molecule, a protein molecule bound to a cell binding ligand, and a support having coupled thereto a plurality of nucleic acid barcode molecules,
- wherein said cell binding ligand comprises a reporter barcode sequence associated with said cell binding ligand, and
- wherein said plurality of nucleic acid barcode molecules comprise:
- (i) a common barcode sequence and (ii) a common capture sequence.
A system for analyzing cells comprising:
- a plurality of partitions comprising a plurality of cells with a plurality of antibodies, wherein a cell of said plurality of cells is bound to an antibody of said plurality of antibodies,
- wherein said antibody is coupled to a reporter oligonucleotide comprising a reporter barcode sequence and a poly-adenine (poly-A) sequence,
- wherein said reporter barcode sequence identifies said antibody,
- wherein said cell comprises a plurality of messenger ribonucleic acid (mRNA) molecules, wherein a partition of said plurality of partitions comprises
- (i) said cell bound to said antibody, and a bead, wherein said bead comprises a plurality of nucleic acid barcode molecules each comprising a common barcode sequence and a poly-thymine (poly-T) sequence.
Dependencies are inferred from the claim text.