Poly(ethylene glycol) brushes for efficient rna delivery
AssigneeNortheastern University
InventorsKe ZHANG, Dali Wang
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Abstract
Disclosed are bottlebrush poly(ethylene glycol) (PEG) polymer-RNA conjugates (pacRNA) having a plurality of PEG side chains attached to a polymer backbone, and one or more RNA oligonucleotides attached to the polymer backbone via a cleavable linkage. The pacRNAs offer potential useful novel RNA-based therapeutics.
Claims20
A bottlebrush poly(ethylene glycol) (PEG) polymer-RNA conjugate (pacRNA) comprising one or more RNA oligonucleotides and a PEG brush polymer comprising a plurality of PEG side chains attached to a polymer backbone, wherein the one or more RNA oligonucleotides are attached to the polymer backbone via a cleavable linkage.
A method of inhibiting expression of a gene product encoded by a target polynucleic acid comprising contacting a cell containing the target polynucleic acid with a bottlebrush poly(ethylene glycol) (PEG) polymer-RNA conjugate (pacRNA) comprising one or more RNA oligonucleotides and a PEG brush polymer comprising a plurality of PEG side chains attached to a polymer backbone to obtain uptake of the pacRNA by the cell, wherein the one or more RNA oligonucleotides are attached to the polymer backbone via a cleavable linkage and wherein the one or more RNA oligonucleotides have a sequence that is complementary to at least a portion of the target polynucleic acid.
A method for promoting cellular uptake of an RNA oligonucleotide, said method comprising contacting a pacRNA with a cell, wherein the pacRNA comprises one or more RNA oligonucleotides and a PEG brush polymer comprising a plurality of PEG side chains attached to a polymer backbone, wherein the one or more RNA oligonucleotides are attached to the polymer backbone via a cleavable linkage.
A composition comprising a (pacRNA) comprising one or more RNA oligonucleotides and a PEG brush polymer comprising a plurality of PEG side chains attached to a polymer backbone, wherein the one or more RNA oligonucleotides are attached to the polymer backbone via a cleavable linkage.
A kit comprising a bottlebrush poly(ethylene glycol) (PEG) polymer-RNA conjugate (pacRNA) comprising one or more RNA oligonucleotides and a PEG brush polymer comprising a plurality of PEG side chains attached to a polymer backbone, wherein the one or more RNA oligonucleotides are attached to thepolymer backbone via a cleavable linkage and wherein the one or more RNA oligonucleotides have a sequence that is complementary to at least a portion of a target polynucleic acid.
Dependencies are inferred from the claim text.