Allergen detection agents and assays
AssigneeDots Technology Corp.
InventorsAdi Gilboa-Geffen, Valerie Villareal, Sarah Stidham, Morris Nehama
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Abstract
The present application discloses nucleic acid aptamer based signaling polynucleotides (SPNs) that specifically bind an allergen protein. Provided in the present invention include aptamers, SPNs, SPN-complement complexes, magnetic particle conjugates, DNA printed glass slides and detection agents, and detection methods using the same for detecting the presence, or absence of an allergen protein in a food sample.
Claims34
-56. (canceled)
An allergen detection agent comprising:
- (a) a solid substrate;
- (b) an allergen binding signaling polynucleotide (SPN) comprising a nucleotide sequence that binds a target allergen and having a 3′ and 5′ end, and
- (c) a complement comprising a nucleotide sequence complementary to a region of the SPN sequence.
A complex for detecting an allergen comprising:
- (a) an allergen binding signaling polynucleotide (SPN) comprising a nucleotide sequence selected from the group consisting of nucleotide sequences presented by SEQ ID NOs. 1-696;
- (b) a complement comprising a nucleotide sequence complementary to a region the SPN sequence wherein the complement comprises 5-20 nucleotide residues;
- and
- (c) a fluorophore.
A method for detecting the absence, presence and/or quantity of an allergen of interest in a sample comprising:
- (a) obtaining and processing a sample suspected to contain the allergen of interest;
- (b) contacting the processed sample with a detection agent;
- (c) shaking the mixture and washing the mixture containing the allergen of interest and the detection agent;
- (d) detecting the SPN-allergen complexes;
- and
- (e) processing and analyzing the detection signals to determine the absence, presence, and/or the quantity of the allergen of interest in the sample,
- wherein the detection agent comprises a signaling polynucleotide (SPN) comprising a nucleotide sequence that specifically binds the allergen of interest, a complement comprising a nucleotide sequence complementary to the SPN sequence, a solid substrate and a fluorophore.
Dependencies are inferred from the claim text.