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US 2019/0292531 A1Published application
Lipase variants, polynucleotides encoding same and the use thereof
AssigneeNovozymes A/S
InventorsMiguel Duarte Guilherme Toscano, Allan Svendsen, Jesper Vind, Alexander David Fulton, Lone Baunsgaard
Life of this patent
Estimating the term…
Abstract
The present invention relates to lipase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims21
A lipase variant of a parent lipase, wherein said variant comprises a modification at one or more positions corresponding to positions 1, 2, 3, 5, 8, 43, 45, 105, 167, 178, 224, 225, 226, 227, 228, 229, 230, 231, 232, 234, 236, 239 and 244 of SEQ ID NO:
- 1, wherein the lipase variant has at least 60% sequence identity, but less than 100% sequence identity, to SEQ ID NO:
- 1;
- the lipase variant has lipase activity;
- and
- a. the modification at the position corresponding to position 1 is a deletion, or an insertion of Arg;
- b. the modification at the position corresponding to position 2 is a deletion;
- c. the modification at the position corresponding to position 3 is a deletion;
- d. the modification at the position corresponding to position 5 is a deletion;
- e. the modification at the position corresponding to position 8 is a substitution with Arg or Lys;
- f. the modification at the position corresponding to position 43 is a deletion;
- g. the modification at the position corresponding to position 45 is a deletion;
- h. the modification at the position corresponding to position 167 is a deletion;
- i. the modification at the position corresponding to position 224 is a deletion, or an insertion of Arg;
- j. the modification at the position corresponding to position 225 is a deletion;
- k. the modification at the position corresponding to position 226 is a deletion, or an insertion of Arg;
- l. the modification at the position corresponding to position 227 is a deletion, or an insertion of Arg;
- m. the modification at the position corresponding to position 228 is a deletion, or an insertion of Arg;
- n. the modification at the position corresponding to position 229 is a deletion, or an insertion of Arg;
- o. the modification at the position corresponding to position 230 is a deletion, or an insertion of Arg, or a substitution with Arg or Lys;
- p. the modification at the position corresponding to position 231 is one or two insertions of Arg;
- q. the modification at the position corresponding to position 232 is an insertion of Arg;
- r. the modification at the position corresponding to position 234 is a deletion;
- s. the modification at the position corresponding to position 236 is a substitution with Arg or Lys;
- t. the modification at the position corresponding to position 239 is a deletion;
- u. the modification at the position corresponding to position 105 is a substitution with Arg, or an insertion of Arg;
- v. the modification at the position corresponding to position 178 is a substitution with Arg;
- and
- w. the modification at the position corresponding to position 244 is an insertion of Arg.
25 more elements
A lipase variant, comprising a modification at two or more positions corresponding to positions 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 15, 28, 29, 30, 31, 33, 37, 38, 39, 40, 42, 43, 45, 73, 91, 99, 101, 102, 105, 106, 167, 178, 180, 192, 193, 194, 199, 200, 202, 210, 211, 217, 218, 220, 221, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 236, 238, 239, 243, 244, 245, 248, 249, 250, 251, 252, 254, and 256 of SEQ ID NO:
- 1, wherein the lipase variant has at least 60% sequence identity, but less than 100% sequence identity, to SEQ ID NO:
- 1;
- the lipase variant has lipase activity;
- and
- a. the modification at the position corresponding to position 1 is a deletion, an insertion of Arg, or a substitution with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- b. the modification at the position corresponding to position 2 is a deletion, or a substitution with Arg or Lys;
- c. the modification at the position corresponding to position 3 is a deletion, or a substitution with Arg or Lys;
- d. the modification at the position corresponding to position 4 is a substitution with Arg or Lys;
- e. the modification at the position corresponding to position 5 is a deletion, or a substitution with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- f. the modification at the position corresponding to position 6 is a substitution with Arg or Lys;
- g. the modification at the position corresponding to position 7 is a substitution with Arg or Lys;
- h. the modification at the position corresponding to position 8 is a substitution with Arg or Lys;
- i. the modification at the position corresponding to position 9 is a substitution with Arg or Lys;
- j. the modification at the position corresponding to position 11 is a substitution with Arg or Lys;
- k. the modification at the position corresponding to position 12 is a substitution with Arg or Lys;
- l. the modification at the position corresponding to position 15 is a substitution with Arg or Lys;
- m. the modification at the position corresponding to position 37 is a substitution with Arg or Lys;
- n. the modification at the position corresponding to position 38 is a substitution with Arg or Lys;
- o. the modification at the position corresponding to position 39 is a substitution with Arg or Lys;
- p. the modification at the position corresponding to position 40 is a substitution with Arg or Lys;
- q. the modification at the position corresponding to position 42 is a substitution with Arg or Lys;
- r. the modification at the position corresponding to position 43 is a deletion, or a substitution at the position corresponding to position 43 with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- s. the modification at the position corresponding to position 45 is a deletion, or a substitution at the position corresponding to position 45 with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- t. the modification at the position corresponding to position 73 is a substitution with Arg or Lys;
- u. the modification at the position corresponding to position 167 is a deletion, or a substitution at the position corresponding to position 167 with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- v. the modification at the position corresponding to position 192 is a substitution with Arg or Lys;
- w. the modification at the position corresponding to position 193 is a substitution with Arg or Lys;
- x. the modification at the position corresponding to position 194 is a substitution with Arg or Lys;
- y. the modification at the position corresponding to position 199 is a substitution with Arg or Lys;
- z. the modification at the position corresponding to position 200 is a substitution with Arg or Lys;
- aa. the modification at the position corresponding to position 202 is a substitution with Arg or Lys;
- ab. the modification at the position corresponding to position 217 is a substitution with Arg or Lys;
- ac. the modification at the position corresponding to position 218 is a substitution with Arg or Lys;
- ad. the modification at the position corresponding to position 220 is a substitution with Arg or Lys;
- ae. the modification at the position corresponding to position 221 is a substitution with Arg or Lys;
- af. the modification at the position corresponding to position 224 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- ag. the modification at the position corresponding to position 225 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- ah. the modification at the position corresponding to position 226 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- ai. the modification at the position corresponding to position 227 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- aj. the modification at the position corresponding to position 228 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- ak. the modification at the position corresponding to position 229 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- al. the modification at the position corresponding to position 230 is a deletion, an insertion of Arg, or a substitution with Arg or Lys;
- am. the modification at the position corresponding to position 231 is one or two insertions of Arg, or a substitution with Arg or Lys;
- an. the modification at the position corresponding to position 232 is an insertion of Arg;
- ao. the modification at the position corresponding to position 233 is a substitution with Arg or Lys;
- ap. the modification at the position corresponding to position 234 is a deletion, or a substitution at the position corresponding to position 234 with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- aq. the modification at the position corresponding to position 236 is a substitution with Arg or Lys;
- ar. the modification at the position corresponding to position 238 is a substitution with Arg or Lys;
- as. the modification at the position corresponding to position 239 is a deletion, or a substitution at the position corresponding to position 239 with Ala, Arg, Asn, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- at. the modification at the position corresponding to position 243 is an insertion of Arg, or a substitution with Arg or Lys;
- au. the modification at the position corresponding to position 244 is an insertion of Arg, or a substitution with Arg or Lys;
- av. the modification at the position corresponding to position 245 is a substitution with Arg or Lys;
- aw. the modification at the position corresponding to position 248 is a substitution with Arg or Lys;
- ax. the modification at the position corresponding to position 249 is a substitution with Arg or Lys;
- ay. the modification at the position corresponding to position 251 is a substitution with Arg or Lys;
- az. the modification at the position corresponding to position 28 is a substitution with Arg;
- ba. the modification at the position corresponding to position 29 is a substitution with Arg;
- bb. the modification at the position corresponding to position 30 is a substitution with Arg;
- bc. the modification at the position corresponding to position 31 is a substitution with Arg;
- bd. the modification at the position corresponding to position 33 is a substitution with Arg;
- be. the modification at the position corresponding to position 99 is a substitution with Arg;
- bf. the modification at the position corresponding to position 101 is a substitution with Arg;
- bg. the modification at the position corresponding to position 102 is a substitution with Arg;
- bh. the modification at the position corresponding to position 105 is a substitution with Arg, or an insertion of Arg;
- bi. the modification at the position corresponding to position 178 is a substitution with Arg;
- bj. the modification at the position corresponding to position 180 is a substitution with Arg;
- bk. the modification at the position corresponding to position 211 is a substitution with Arg;
- bl. the modification at the position corresponding to position 250 is a substitution with Arg;
- bm. the modification at the position corresponding to position 252 is a substitution with Arg;
- bn. the modification at the position corresponding to position 254 is a substitution with Arg;
- and
- bo. the modification at the position corresponding to position 256 is a substitution with Arg.
69 more elements
(canceled)
17-18. (canceled)
Dependencies are inferred from the claim text.