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US 2019/0292518 A1Published application
Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof
AssigneeHoffmann-La Roche Inc.
InventorsEva Christina THOMA, Martin GRAF, Vanessa HUNT
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Abstract
This application relates to non-human primate (NHP) induced pluripotent stem cell (IPSC)-derived hepatocytes and methods of producing the same. Moreover, this application relates to methods of using NHP IPSC-derived hepatocytes for drug screening, drug safety assessment and in models of infection.
Claims21
A method for differentiating non-human primate (NHP) pluripotent stem cells into NHP hepatocytes, the method comprising:
- a) providing NHP pluripotent stem cells in a feeder-free culture in a chemically defined medium;
- b) contacting the pluripotent stem cells with a Wnt signalling activator to produce endodermal cells;
- c) contacting the endodermal cells with BMP4 and a fibroblast growth factor to produce immature NHP hepatocytes;
- and
- d) contacting the immature NHP hepatocytes with HGF, OncostatinM and Dexamethasone to produce NHP hepatocytes.
2 more elements
A feeder-free NHP hepatocyte culture in a chemically defined medium.
(canceled)
Dependencies are inferred from the claim text.