Cell expressing car and gpcr

Abstract

The present invention provides a cell which co-expresses (i) a chimeric antigen receptor (CAR) and a GPCR at the cell surface and (ii) an intracellular component comprising a protease domain linked to a GPCR-targeting domain; wherein an intracellular domain of the GPCR comprises a protease cleavage site linked to a transcriptional regulatory domain, which transcriptional regulatory domain is capable of modulating the expression of a nucleic acid encoding the CAR; and wherein the protease domain of the intracellular component is capable of cleaving the cleavage site; such that, upon binding of ligand to the GPCR, the intracellular component is recruited to the GPCR and the protease domain cleaves the cleavage site thereby releasing the transcriptional regulatory domain from the GPCR.

Dependencies are inferred from the claim text.