Human placental tissue graft products, methods, and apparatuses
AssigneeCryolife, Inc.
InventorsSteven GOLDSTEIN, Adam MARTINEZ, Candace LAW
Life of this patent
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Abstract
Provided herein are tissue grafts, and in particular human placenta-derived tissue grafts and methods and articles for the manufacture and use thereof.
Claims39
1-15. (canceled)
A tissue graft comprising:
- a processed human fetal amniotic membrane comprising an amnion layer and a chorion layer wherein processing the human fetal amniotic membrane comprises:
- removing maternal decidua cells from the membrane;
- compressing the membrane;
- and
- dehydrating the membrane,
- wherein processing the membrane provides a dense matrix having a compact structure and a reduced amount of maternal decidua cells without a step of delaminating the amnion layer from the chorion layer.
17-22. (canceled)
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26-31. (canceled)
33-42. (canceled)
44-52. (canceled)
55-57. (canceled)
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61-74. (canceled)
The tissue graft of claim 17, wherein the compressing the membrane step and the dehydrating the membrane step produces visibly distinguishable surfaces of the membrane.
76-77. (canceled)
79-85. (canceled)
87-97. (canceled)
99-104. (canceled)
106-131. (canceled)
A method of treating a wound on a patient comprising:
- applying to the wound, a tissue graft comprising a human fetal amniotic membrane comprising an amnion layer and a chorion layer wherein the two layers have not been delaminated,
- wherein the membrane is prepared by a process comprising
- maintaining the membrane so that the amnion and chorion layers are not delaminated;
- removing maternal decidua cells from the membrane;
- and
- compressing and dehydrating the membrane to result in a dense and compact matrix.
133-135. (canceled)
An apparatus for compressing and dehydrating a tissue, comprising:
- a chamber defining an opening at one end, the chamber being in fluid connection with a vacuum source;
- a support platform covering the opening of the chamber;
- and
- a sealing sheet positioned adjacent the perforated support platform opposite the chamber, the sealing sheet being configured to seal the chamber upon depressurization of the chamber by the vacuum source,
- wherein the apparatus is configured to receive the tissue between the support platform and the sealing sheet, such that the tissue is dehydrated upon depressurization of the chamber.
138-140. (canceled)
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144-145. (canceled)
147-148. (canceled)
Dependencies are inferred from the claim text.