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US 2019/0290730 A1Published application
Methods and compositions for stimulation of cell proliferation and provision of biologically active mixtures of fgf2 isoforms
AssigneeSanBio, Inc.
InventorsIrina AIZMAN, Damien BATES
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Abstract
Disclosed herein are methods and compositions for providing mixtures of FGF2 isoforms that are biologically active. The biological activities include, but are not limited to, stimulation of proliferation of neural precursor cells, stimulation of proliferation of endothelial cells, stimulation of development of neural precursor cells, and stimulation of development of astrocytes.
Claims21
A method for inducing proliferation of a neural precursor cell (NPC);
- the method comprising providing FGF2 to the neural precursor cell;
- wherein the FGF2 is present in a soluble, cell-free extract of a descendant of a mesenchymal stem cell (MSC) that has been transiently transfected with a vector comprising sequences encoding a Notch intracellular domain (NICD).
A method for inducing proliferation of an endothelial cell;
- the method comprising providing FGF2 to the endothelial cell;
- wherein the FGF2 is present in a soluble, cell-free extract of a descendant of a mesenchymal stem cell (MSC) that has been transiently transfected with a vector comprising sequences encoding a Notch intracellular domain (NICD).
A method for stimulating proliferation of a neural precursor cell or an endothelial cell;
- the method comprising contacting the neural precursor cell or the endothelial cell with a combination comprising:
- (a) FGF-2, and
- (b) conditioned medium from a descendant of a mesenchymal stem cell (MSC) that has been transiently transfected with a vector comprising sequences encoding a Notch intracellular domain (NICD).
Dependencies are inferred from the claim text.