Isolation, cultivation and uses of stem/progenitor cells
AssigneeCellresearch Corporation Pte. Ltd.
InventorsToan Thang PHAN, Ivor Jiun LIM
Life of this patent
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Abstract
The present invention relates to an epithelial stem/progenitor cell population isolated from the amniotic membrane of umbilical cord, the epithelial stem/progenitor cell population having the capacity to differentiate in multiple cell types. The invention also relates to a pharmaceutical composition comprising such an epithelial stem/progenitor cell population or a cellular extract thereof.
Claims9
An epithelial stem/progenitor cell population isolated from the amniotic membrane of umbilical cord, the epithelial stem/progenitor cell population having the capacity to differentiate in multiple cell types and being isolated by a method comprising:
- (a) separating the amniotic membrane from the other components of the umbilical cord in vitro;
- (b) culturing the amniotic membrane tissue obtained in (a) as a tissue explant, or culturing amniotic membrane cells separated from the amniotic membrane tissue obtained in (a), under conditions allowing cell proliferation of epithelial stem/progenitor cells without differentiation of the epithelial stem/progenitor cells;
- and
- (c) harvesting the epithelial stem/progenitor cells,
- wherein the epithelia stem/progenitor cells have a polyhedral shape, express the following genes:
- POU5f1, Bmi-1, leukemia inhibitory factor (LIF), and wherein the epithelial stem/progenitor cells secret Activin A and Follistatin.
Dependencies are inferred from the claim text.