Humanized anti-muc1* antibodies and use of cleavage enzyme

An immune cell transfected or transduced with a cleavage enzyme for the treatment of cancer.

An immune cell transfected or transduced with both a CAR comprising an antibody fragment, and a cleavage enzyme or catalytically active fragment thereof for the treatment of cancer.

(canceled)

A nucleic acid construct comprising nucleic acid encoding a cleavage enzyme or catalytically active fragment thereof located downstream of an NFAT promoter.

The nucleic acid construct comprising nucleic acid encoding a cleavage enzyme or catalytically active fragment thereof located downstream of at least one NFAT response element.

A method of pre-activating an immune cell transduced or transfected with nucleic acid encoding CAR that is specific for MUC1* and/or MUC1 specific cleavage enzyme by co-culturing the immune cell in vitro on a surface that presents a peptide having the sequence of truncated extracellular domain of MUC1, thus obtaining pre-activated immune cell.

An immune cell transfected or transduced with a plasmid encoding a CAR and a plasmid encoding a non-CAR species that is expressed from an inducible promoter.