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US 2019/0290248 A1Published application
Sweat as a biofluid for analysis and disease identification
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsBenjamin KATCHMAN, Karen ANDERSON, Jennifer Blain CHRISTEN
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Abstract
Provided herein are methods of detecting and measuring disease-associated analytes such as proteins and antibodies in a sweat sample. Also provided are methods of determining and monitoring the disease state or physiological condition of a subject using small sweat samples.
Claims22
A method for determining a concentration of an analyte in sweat, comprising the steps of:
- providing between about 1 μl and about 50 μl of a body fluid sample comprising sweat, wherein the sample is collected using a wearable sample collection device;
- contacting at least a portion of the collected sample to an analyte measurement device comprising an assay unit, wherein the assay unit is configured to detect a plurality of analytes and comprises reactants for detection of the plurality of analytes, and wherein the wearable sample collection device and the analyte measurement device comprise a single integrated device;
- allowing the collected body fluid sample to react with the reactants, whereby a detectable reaction product is produced if a target analyte is present in the sample;
- and
- detecting and measuring the reaction product, wherein the presence of detectable reaction product indicates the presence of the target analyte in the sample;
- and wherein the amount of detectable reaction product is related to the concentration of the target analyte in the sample.
3 more elements
A method of monitoring changes in an individual's health state over time, the method comprising
- (a) detecting the presence of one or more target analytes in a first sweat sample from the subject, wherein detecting comprises:
- (i) collecting between about 5 μL to about 20 μL of a body fluid sample comprising sweat, wherein the sample is collected using a wearable sample collection device;
- (ii) contacting at least a portion of the collected sample to an analyte measurement device comprising an assay unit, wherein the assay unit is configured to detect a plurality of analytes and comprises reactants for detection of the plurality of analytes, and wherein the wearable sample collection device and the analyte measurement device comprise a single integrated device;
- (iii) allowing the collected body fluid sample to react with the reactants, whereby a detectable reaction product is produced if a target analyte is present in the sample;
- and
- (iv) detecting the reaction product, wherein the presence of detectable reaction product indicates the presence of the target analyte in the sample;
- (b) measuring a concentration of the detected target analyte of the first sweat sample to establish a baseline level of said target analyte, wherein the amount of detectable reaction product is related to the concentration of the target analyte in the sample;
- (c) repeating steps (a) and (b) to detect and measure the concentration of target analyte in a second sweat sample obtained from the individual at after a predetermined interval of time;
- (d) comparing the concentrations measured for the first and second sweat samples to detect a positive or negative change in the concentrations as an indicator of a positive or negative change in the individual's health state over the predetermined interval of time.
6 more elements
(canceled)
A method of detecting a disease-specific antibody in a body fluid sample, the method comprising:
- collecting between about 5 μL to about 20 μL of a body fluid sample comprising sweat, wherein the sample is collected using a wearable sample collection device;
- contacting at least a portion of the collected sample to an analyte measurement device comprising an assay unit, wherein the assay unit is configured to detect at least one disease-specific antibody and comprises reactants for detection of the at least one disease-specific antibody, and wherein the wearable sample collection device and the analyte measurement device comprise a single integrated device;
- allowing the collected body fluid sample to react with the reactants, whereby a detectable reaction product is produced if a target disease-specific antibody is present in the sample;
- and
- detecting and measuring the reaction product, wherein the presence of detectable reaction product indicates the presence of the target disease-specific antibody in the sample;
- and wherein the amount of detectable reaction product is related to the concentration of the target disease-specific antibody in the sample.
3 more elements
(canceled)
Dependencies are inferred from the claim text.