Mixotrophic Chlorella-Based Composition, and Methods of its Preparation and Application to Plants
AssigneeHeliae Development, LLC
InventorsSandip Shinde, Stephen Ventre, Nicholas Donowitz, Michael Rohlfsen, Laura Carney
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Abstract
Methods preparing a liquid mixotrophic Chlorella based composition comprising pasteurization and stabilization of a low concentration of mixotrophic Chlorella whole cells are disclosed. The liquid composition can be used to enhance the emergence and growth of plants in low concentration and low frequency soil, foliar, seed soak, and hydroponic applications.
Claims40
A method of preparing a whole cell Chlorella liquid plant growth composition to make them suitable in enhancing the growth of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells, comprising:
- heating a culture comprising whole Chlorella cells in a liquid culture medium;
- cooling the heated composition;
- wherein the heating and cooling steps pasteurize the whole Chlorella cells in the composition, making them suitable in the enhancement of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells.
A method of preparing a whole cell Chlorella liquid plant growth composition to make them suitable in enhancing the growth of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells, comprising:
- heating a culture comprising whole Chlorella cells in a liquid culture medium at a temperature in the range of 50-90° C.;
- cooling the heated composition to a temperature in the range of 35-45° C.;
- wherein the heating and cooling steps pasteurize the whole Chlorella cells in the composition, making them suitable in the enhancement of a plant compared to a composition comprising non-pasteurized Chlorella cells, lacking the whole pasteurized Chlorella cells.
Dependencies are inferred from the claim text.