Piv5 as an oncolytic agent
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorBiao He
Life of this patent
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Abstract
The present invention includes the Paramyxovirus Parainfluenza Virus 5 (PIV5) as an oncolytic agent for treating various cancers, including, but not limited to breast cancer, lung cancer and melanoma. PIV5 oncolytic agents include both wild type PIV5 and various recombinant PIV5 constructs. Recombinant PIV5 constructs may include PIV5 lacking the conserved C-terminus of the V protein (PIV5VΔC), PIV5 with mutations in the N-terminus of the V/P protein (PIV5CPI−), and PIV5 expressing MDA-7/IL-24 (rPIV5-MDA7), rPIV5-V/P-CPI−, rPIV5-CPI+, rPIV5-Rev, rPIV5-RL, rPIV5-P-S157A, rPIV5-P-S308A, rPIV5-L-A1981D, rPIV5-F-S443P, rPIV5-MDA7, rPIV5ΔSH-CPI−, or rPIV5ΔSH-Rev. Also included are methods of making and using such oncolytic agents and compositions including such oncolytic agents.
Claims17
A method of treating a subject with a cancer, the method comprising administering to the subject an effective amount of a composition comprising an isolated recombinant parainfluenza virus 5 (PIV5), wherein the PIV5 comprises one or more mutations.
A method of imaging a tumor in a subject, the method comprising administering to the subject a recombinant parainfluenza virus 5 (PIV5) expressing a fluorescent polypeptide or detectable agent.
An oncolytic agent comprising a mutant parainfluenza virus 5 (PIV5) comprising one or more the mutations selected from the group consisting of a mutation of the V/P gene, a mutation of the shared N-terminus of the V and P proteins, a mutation of residues 26, 32, 33, 50, 102, and/or 157 of the shared N-terminus of the V and P proteins, a mutation lacking the C-terminus of the V protein, a mutation lacking the small hydrophobic (SH) protein, a mutation of the fusion (F) protein, a mutation of the phosphoprotein (P), a mutation of the large RNA polymerase (L) protein, a mutation incorporating residues from canine parainfluenza virus, and/or a mutation that enhances synctial formation.
Dependencies are inferred from the claim text.