Carbohydrate-enriched recombinant microorganisms

A recombinant methanotrophic bacterium, comprising an exogenous nucleic acid that encodes a gluconeogenesis enzyme,

• wherein the encoded gluconeogenesis enzyme is one or more of a pyruvate carboxylase, a phosphoenolpyruvate carboxykinase, an enolase, a phosphoglycerate mutase, a phosphoglycerate kinase, a glyceraldehyde-3-phosphate dehydrogenase, a Type A aldolase, a fructose 1,6-bisphosphatase, a phosphofructokinase, a phosphoglucose isomerase, a hexokinase, and a glucose-6-phosphate,
• wherein the methanotrophic bacterium is selected from the group consisting of Methylomonas, Methylobacter, Methylococcus, Methylosinus, Methylocystis, Methylomicrobium, Methanomonas, and Methylocella, and
• wherein the recombinant methanotrophic bacterium grown on a C1 substrate feedstock is capable of producing glucose at a level that is greater than that produced by the parent methanotrophic bacterium.