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US 2019/0271009 A1Published application
Methods, cells and reagents for production of isoprene, derivatives and intermediates thereof
OwnerAlex Van Eck Conradieinventor
Life of this patent
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Abstract
This application describes methods, including non-naturally occurring methods, for biosynthesizing 3-hydroxy-3-methylglutaryl-coA and intermediates thereof, as well as non-naturally occurring hosts for producing 3-hydroxy-3-methylglutaryl-coA. This application also describes methods, including non-naturally occurring methods, for biosynthesizing isoprene and intermediates thereof, as well as non-naturally occurring hosts for producing isoprene.
Claims20
-10. (canceled)
A non-naturally occurring host capable of producing 3-hydroxy-3-methylglutaryl-CoA, said host comprising:
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 1.3.8.4 enzyme;
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 6.4.1.4 enzyme;
- and
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 4.2.1.18 enzyme;
- and wherein said host further comprises:
- (a) at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 1.2.7.7 or EC 1.2.1.-enzyme;
- (b) at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 4.1.1.74 or EC 41.2.1.43 enzyme,
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 1.2.1.39 or EC 1.2.1.5 enzyme, and
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 6.2.1.2. enzyme;
- or
- (c) at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 1.2.7.7 or EC 1.2.1.-enzyme,
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 4.1.1.74 or EC 41.2.1.43 enzyme,
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 1.2.1.39 or EC 1.2.1.5 enzyme, and
- at least one exogenous nucleic acid encoding a polypeptide having the activity of an EC 6.2.1.2. enzyme.
11 more elements
-13. (canceled)
-19. (canceled)
(canceled)
-42. (canceled)
Dependencies are inferred from the claim text.