N-acetyl homoserine
AssigneeEvonik Operations GmbH
InventorsThomas HAAS, Steffen SCHAFFER, Thomas BÜLTER, Simon BECK
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Abstract
The present invention relates to a compound of general formula I The present invention also relates to a method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of (a) an enzyme E1, a homoserine dehydrogenase (EC1.1.1.3) and/or an enzyme E5, an aspartokinase (EC2.7.2.4); and (b) an enzyme E2, a homoserine O-acetyl transferase (EC2.3.1.31) and the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.
Claims21
1-15. (canceled)
A compound of general formula I:
The compound of claim 1, wherein the compound is O-acetyl-N-acetamido-L-homoserine or O-acetyl-N-acetamido-D-homoserine.
A method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of:
- (a) an enzyme E1, a homoserine dehydrogenase (EC 1.1.1.3) and/or an enzyme E5, an aspartokinase (EC2.7.2.4);
- and
- (b) an enzyme E2, a homoserine O-acetyl transferase (EC2.3.1.31)
- and wherein the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.
The method of 33, wherein the recombinant cell comprises increased activity of enzymes E1, E5 and E2 relative to the wild type cell.
Dependencies are inferred from the claim text.