Compositions And Methods For Production Of Salidroside, Icariside D2, And Precursors Of Salidroside And Icariside D2
AssigneeWhitehead Institute for Biomedical Research
InventorsMichael Torrens-Spence, Jing-Ke Weng
Life of this patent
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Abstract
Transgenic host cells, vectors useful for making transgenic host cells, and kits useful for making transgenic host cells are described. Also described are transgenic plants. In some embodiments, transgenic host cells express a 4-hydroxyphenylacetaldehyde synthase (4HPAAS). In some embodiments, transgenic host cells express a tyrosol:UDP-glucose 8-O-glucosyltransferase (T8GT). The transgenic host cells are useful for biosynthesis of one or more of salidroside, icariside D2, tyrosol, and 4-hydroxypenylacetaldehyde.
Claims29
1-79. (canceled)
A host cell comprising a transgene encoding a 4-hydroxyphenylacetaldehyde synthase (4HPAAS), wherein the 4HPAAS comprises an amino acid sequence having at least 70% amino acid sequence identity to SEQ ID NO:
- 2, and wherein the amino acid sequence of the 4HPAAS comprises:
- a) an amino acid residue selected from the group consisting of F, L, I, M and V at a position corresponding to the F residue at position 343 in SEQ ID NO:
- 2;
- b) an amino acid residue selected from the group consisting of N and D at a position corresponding to the H residue at position 198 in SEQ ID NO:
- 2;
- or
- c) a combination thereof.
84-86. (canceled)
88-108. (canceled)
111-187. (canceled)
A method of making tyrosol 8-O-glucoside (salidroside) in a host cell, the method comprising expressing in a host cell a transgene that encodes a tyrosol:UDP-glucose 8-O-glucosyltransferase (T8GT), wherein the T8GT comprises an amino acid sequence having at least 70% amino acid sequence identity to one or more of SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 18, and SEQ ID NO:
- 20, wherein the host cell synthesizes tyrosol.
190-215. (canceled)
218-220. (canceled)
A method of making 4-hydroxyphenylacetaldehyde (4-HPAA) in a host cell, the method comprising expressing in the host cell a transgene that encodes a 4-hydroxyphenylacetaldehyde synthase (4HPAAS), wherein the 4HPAAS comprises an amino acid sequence having at least 70% sequence identity to SEQ ID NO:
- 2, and wherein the amino acid sequence of the 4HPAAS comprises:
- a) an amino acid residue selected from the group consisting of F, L, I, M and V at a position corresponding to the F residue at position 343 in SEQ ID NO:
- 2;
- b) an amino acid residue selected from the group consisting of N and D at a position corresponding to the H residue at position 198 in SEQ ID NO:
- 2;
- or
- c) a combination thereof.
226-228. (canceled)
231-235. (canceled)
238-249. (canceled)
Dependencies are inferred from the claim text.