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US 2019/0264170 A1Published application
Culture medium protective liquid material and embryo culture method
AssigneeKitazato Corporation
InventorFutoshi INOUE
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Abstract
A culture medium protective liquid material of the present invention covers a culture medium when a living cell is cultured. The culture medium protective liquid material contains liquid paraffin which is liquid at room temperature as a main component thereof and an antioxidative substance for restraining the liquid paraffin from being oxidized.
Claims16
A culture medium protective liquid material for covering a culture medium when a living cell is cultured,
- said culture medium protective liquid material containing liquid paraffin which is liquid at room temperature as a main component thereof and an antioxidative substance for restraining said liquid paraffin from being oxidized.
An embryo culture method comprising
- a step of dispensing a culture medium to a container in a layer form,
- a step of putting an embryo into said culture medium,
- a step of covering with a culture medium protective liquid material to an exposed surface of said culture medium including said embryo,
- a step of culturing said embryo in said culture medium covered with said culture medium protective liquid material, and
- wherein said culture medium protective liquid material containing liquid paraffin which is liquid at room temperature as a main component thereof and an antioxidative substance for restraining said liquid paraffin from being oxidized,
- said antioxidative substance is vitamin E,
- said culture medium protective liquid material is free of aromatic hydrocarbons and free of sulfur compounds,
- an amount of said antioxidative substance to be added to 100 parts by weight of said liquid paraffin is 0.005 to 0.1 parts by weight,
- wherein said liquid paraffin has an average molecular weight of 270 to 330, a kinematic viscosity of 5-15 mm2/s at 40 degrees C., and a specific gravity of 0.82 to 0.85.
6 more elements
An embryo culture method comprising
- a step of forming a droplet-shaped culture medium on a bottom surface of a container,
- a step of putting an embryo into said droplet-shaped culture medium,
- a step of covering with a culture medium protective liquid material to an exposed surface of said droplet-shaped culture medium including said embryo,
- a step of culturing said embryo in said culture medium covered with said culture medium protective liquid material, and
- wherein said culture medium protective liquid material containing liquid paraffin which is liquid at room temperature as a main component thereof and an antioxidative substance for restraining said liquid paraffin from being oxidized,
- said antioxidative substance is vitamin E,
- said culture medium protective liquid material is free of aromatic hydrocarbons and free of sulfur compounds,
- an amount of said antioxidative substance to be added to 100 parts by weight of said liquid paraffin is 0.005 to 0.1 parts by weight,
- said liquid paraffin has an average molecular weight of 400 to 450, a kinematic viscosity of 35 to 45 mm2/s at 40 degrees C., and a specific gravity of 0.85 to 0.88.
6 more elements
Dependencies are inferred from the claim text.