Reaction Vessel Holder And Molecule Detection Device

A device for molecule analysis of a sample in a reaction vessel, the device comprising:

• a reaction vessel holder for receiving the reaction vessel, the reaction vessel for containing the sample and having at least one portion that is substantially optically transparent to light of at least a first range of wavelengths, the reaction vessel holder comprising a body arranged to thermally couple to and support the reaction vessel,
• a thermal device, thermally coupled to the body, that heats and/or cools the reaction vessel holder and thereby the reaction vessel,
• an excitation arrangement that generates one or more excitation beams to stimulate an emission of reaction light from the sample;
• and
• a detector arrangement that detects the reaction light from the sample,
• wherein the body comprises at least one transparent portion that is substantially optically transparent to the light of the at least a first range of wavelengths, such that the optically transparent portion of the reaction vessel is adapted to face the transparent portion of the body such that light of the first range of wavelengths to and/or from the sample in the reaction vessel pass through the transparent portion of the reaction vessel,
• and wherein the excitation arrangement and the detector arrangement are positioned at or facing opposite sides of the reaction vessel such that an optical path of the excitation arrangement and an optical path of the detector arrangement are substantially collinear or parallel.

A method for detection of one or more molecules in a sample contained in a reaction vessel, comprising:

• providing a reaction vessel having a portion that is substantially optically transparent to a light of at least a first range of wavelengths,
• thermally coupling the reaction vessel to a reaction vessel holder comprising a body arranged to thermally couple to and support the reaction vessel, the body further comprising at least one transparent portion that is substantially optically transparent to the light of the at least a first range of wavelengths, such that the optically transparent portion of the reaction vessel is adapted to face the transparent portion of the body such that light of the first range of wavelengths to and/or from the sample in the reaction vessel pass through the transparent portion of the reaction vessel;
• and
• guiding light to and/or from the sample in the reaction vessel through the transparent portion of the reaction vessel holder and through the transparent portion of the reaction vessel.