Silane mixtures
AssigneeAffymertix, Inc.
InventorsGlenn H. McGall, Robert G. Kuimelis
Life of this patent
Estimating the term…
Abstract
Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.
Claims20
A method of preparing an array of polymers comprising:
- providing a substrate;
- contacting a silica or other silicon oxide-containing surface of the substrate with a silanation composition that comprises a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis;
- protecting the functional group capable of supporting polymer synthesis with a protecting group or reacting the functional group capable of supporting polymer synthesis with a compound having a reactive group protected by a protecting group;
- removing the protecting group in selected regions of the surface to provide an exposed functional or reactive group;
- reacting said exposed group with a monomer, wherein the monomer is coupled to the exposed group;
- and
- repeating the steps of removing and reacting to produce the array of polymers.
A method of preparing an array of polymers according to 8, wherein said predetermined ratios are molar ratios.
Dependencies are inferred from the claim text.