Chimeric dengue virus e glycoproteins comprising mutant domain i and domain ii hinge regions
AssigneesThe University of North Carolina at Chapel Hill, Vanderbilt University
InventorsAravinda M. de Silva, A. Ruklanthi de Alwis, Wahala M.P.B. Wahala, Ralph S. Baric, William B. Messer, James E. Crowe, JR., Scott A. Smith
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Abstract
The present invention provides compositions and methods of use comprising a chimeric dengue virus E glycoprotein comprising a dengue virus E glycoprotein backbone, which comprises amino acid substitutions that introduce a dengue virus E glycoprotein domain I and domain II hinge region from a dengue virus serotype that is different from the dengue virus serotype of the dengue virus E glycoprotein backbone.
Claims17
(canceled)
A chimeric dengue virus E glycoprotein comprising a dengue virus E glycoprotein backbone from dengue virus serotype 2 that comprises the following amino acid substitutions that introduce an epitope that is recognized by an antibody that is reactive with a dengue virus serotype that is different from the dengue virus serotype of the dengue virus E glycoprotein backbone;
- wherein the amino acid residue numbering is based on the amino acid sequence of SEQ ID NO:20:
- Q52E, P53V, T55L, K58T, N124K, M1251, E126T, K128N, V129L, Q200K, E202K, N203K, A205T, R210K, L221T, P222A, Q271D, M272S, S273G, S274D, L277H, L278M, and T280A.
A chimeric dengue virus E glycoprotein comprising a dengue virus E glycoprotein backbone from dengue virus serotype 3 that comprises the following amino acid substitutions that introduce an epitope that is recognized by an antibody that is reactive with a dengue virus serotype that is different from the dengue virus serotype of the dengue virus E glycoprotein backbone;
- wherein the amino acid residue numbering is based on the amino acid sequence of SEQ ID NO:17:
- N270T, G273T, and S275T.
Dependencies are inferred from the claim text.