Fusion polypeptides
AssigneeOxford University Innovation Limited
InventorsDavid WYLLIE, Pauline VAN DIEMEN, Christine ROLLIER
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Abstract
The present invention relates to fusion polypeptides comprising (a) a 4-oxalocrotonate tautomerase (4-OT)-based polypeptide scaffold which is capable of forming multimers, and (b) a polypeptide antigen; and homo- and hetero-multimers thereof. The invention also provides nucleic acid molecules and vectors encoding the fusion polypeptides and multimers; and methods of using the fusion polypeptides, multimers, nucleic acid molecules and vectors to produce an immunogenic response against the polypeptide antigen.
Claims33
A fusion polypeptide, wherein the fusion polypeptide comprises:
- (a) a 4-oxalocrotonate tautomerase (4-OT)-based polypeptide scaffold which is capable of forming multimers;
- and
- (b) a polypeptide antigen.
A fusion polypeptide as claimed in any one of the preceding claims, wherein the polypeptide antigen is one which is presented on a microbe, parasite or neoplasm.
A fusion polypeptide as claimed in any one of the preceding claims, wherein the polypeptide antigen is a viral, bacterial, protozoan, animal, mammalian or human antigen.
A fusion polypeptide as claimed in any one of the preceding claims, wherein the polypeptide antigen is from a disease-causing bacterium, a disease-causing parasite or a disease-causing virus, preferably from a malaria-causing parasite or an influenza-causing virus.
A fusion polypeptide as claimed in any one of the preceding claims, wherein the polypeptide antigen is a bacterial or parasitic antigen which is from or derived from Staphylococcus, pathogenic Neisseria, Mycobacteria, Escherichia or from Apicomplexa (e.g. Plasmodium) or helminths, preferably from or derived from S. aureus, a pathogenic Neisseria species, M. tuberculosis, E. coli or P. falciparum.
A fusion polypeptide as claimed in any one of the preceding claims, wherein the polypeptide antigen is selected from the group consisting of ClfA, ClfB, FnBPA, FnBP, SdrC, SdrD, SdrE, SasA, SasB, SasC, SasD, SasX, SasF, SasG/AAp, MntC, IsdA, IsdB, IsdH, FhuD2, EsxA, EsxB, Spa, Coa, vWbp, HIa, HIgA, HIgB, HIgC, LukA, LukB, LukD, LukE, EpiP, Can, Csa1A, Csa1B, Csa1C, Csa1D, CsA2A, Csa3A, Csa3B, CsA3C, Csa3D, Csa3E, Csa3G, Csa3H, Csa31, Csa3J, Csa4A, Csa4B, Csa4c, scn, efb, efbc, or a variant or derivative thereof which maintains the immunogenic capacity of the antigen.
Dependencies are inferred from the claim text.