Maltooligosyl Trehalose Trehalohydrolase (MTHase) Mutant and Application Thereof
AssigneeJiangnan University
InventorsJing WU, Lingqia SU, Zhendong WANG
Life of this patent
Estimating the term…
Abstract
The present invention discloses a maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application thereof, belonging to the technical fields of gene engineering and enzyme engineering. The present invention provides a series of MTHase mutants to prepare trehalose, having a better effect. Further, the MTHase mutant is expressed in Escherichia coli BL21 (DE3) and the enzyme is optimized by fermentation, which can significantly increase the yield of enzymes.
Claims10
A mutant of a maltooligosyl trehalose trehalohydrolase, comprising an amino acid sequence with mutations selected from the group consisting of mutation of methionine (Met) at position 140, mutation of leucine (Leu) at position 202, mutation of leucine (Leu) at position 218, mutation of tyrosine (Tyr) at position 323, mutation of phenylalanine (Phe) at position 338 and mutation of isoleucine (Ile) at position 404;
- wherein the mutations are relative to a parent amino acid sequence of a maltooligosyl trehalose trehalohydrolase derived from Sulfolobus acidocaldarius set forth in SEQ ID NO.2.
Dependencies are inferred from the claim text.