Processes to prepare elongated 2-ketoacids and c5-c10 compounds therefrom via genetic modifications to microbial metabolic pathways
AssigneeDow Global Technologies LLC
InventorsParesh C. Sanghani, Sarah Delaplane
Life of this patent
Estimating the term…
Abstract
Genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids utilizing genetically modified isopropylmalate synthases, and microbial organisms including genetically modified isopropylmalate synthases are described. The genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids, and microbial organisms including genetically modified isopropylmalate synthases can be particularly useful for producing corresponding Cn_1 aldehydes, alcohols, carboxylic acids, and Cn_2 alkanes both in vivo and in vitro.
Claims14
1-21. (canceled)
A process for preparing a C7-C11 2-ketoacid, the process comprising:
- (I) providing at least one of a C4-C10 2-ketoacid substrate, with:
- (a) a genetically modified isopropylmalate synthase (IPMS) comprising at least one of:
- (i) an amino acid sequence comprising SEQ ID NO:
- 1 and comprising the mutations H97A, S139G, N167G, P169A, G181A, A182G, G210A, A214S, and G462D and having IPMS activity;
- (ii) an amino acid sequence comprising SEQ ID NO:
- 2 and comprising the mutations H97A, S139G, N167G, P169A, M255L, R260A, N264Q, and G462D and having IPMS activity;
- or
- (iii) an amino acid sequence comprising SEQ ID NO:
- 2 and comprising the mutations H97A, S139G, N167G, P169A, D348E, D350E, M353L, Q355N, and G462D and having IPMS activity;
- (b) a isopropylmalate isomerase having isopropylmalate isomerase activity;
- and
- (c) a isopropylmalate dehydrogenase having isopropylmalate dehydrogenase activity;
- under conditions that the at least one of the C4-C10 2-ketoacid substrate is converted to the C7-C11 2-ketoacid;
- and wherein the conversion of the at least one of the C4-C10 2-ketoacid substrate to the C7-C11 2-ketoacid occurs via one or more biochemical reactions.
A microbial organism comprising at least one of:
- (a) a genetically modified isopropylmalate synthase (IPMS) comprising an amino acid sequence of SEQ ID NO:
- 1 and comprising the mutations H97A, S139G, N167G, P169A, G181A, A182G, G210A, A214S, and G462D and having IPMS activity;
- (b) a genetically modified IPMS comprising an amino acid sequence of SEQ ID NO:
- 2 and comprising the mutations H97A, S139G, N167G, P169A, M255L, R260A, N264Q, and G462D and having IPMS activity;
- or
- (c) a genetically modified IPMS comprising an amino acid sequence of SEQ ID NO:
- 2 and comprising the mutations H97A, S139G, N167G, P169A, D348E, D350E, M353L, Q355N, and G462D and having IPMS activity.
A genetically modified isopropylmalate synthase polypeptide having isopropylmalate activity, the polypeptide comprising at least one of:
- (a) an amino acid sequence of SEQ ID NO:
- 1 and comprising the mutations H97A, S139G, N167G, P169A, G181A, A182G, G210A, A214S, and G462D;
- (b) an amino acid sequence of SEQ ID NO:
- 2 and comprising the mutations H97A, S139G, N167G, P169A, M255L, R260A, N264Q, and G462D;
- or
- (c) an amino acid sequence of SEQ ID NO:
- 2 and comprising the mutations H97A, S139G, N167G, P169A, D348E, D350E, M353L, Q355N, and G462D.
Dependencies are inferred from the claim text.