Back to results
US 2019/0241622 A1Published application
Protein Secretory Production Method
AssigneeAjinomoto Co., Inc.
InventorsYumi Ito, Yoshihiko Matsuda
Life of this patent
Estimating the term…
Abstract
A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium able to secrete a heterologous protein and modified so that the activity of HrrSA system is reduced is cultured to produce the heterologous protein by secretory production.
Claims23
A method for producing a heterologous protein comprising:
- culturing a coryneform bacterium having a genetic construct for secretory expression of the heterologous protein;
- and
- collecting the heterologous protein produced by secretory production,
- wherein the coryneform bacterium has been modified so that the number of molecules of a HrrSA system per cell is reduced as compared with a non-modified strain,
- wherein the genetic construct comprises, in the direction from 5′ to 3′, a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence encoding a signal peptide that functions in the coryneform bacterium, and a nucleic acid sequence encoding the heterologous protein, and
- wherein the heterologous protein is expressed as a fusion protein with the signal peptide.
3 more elements
Dependencies are inferred from the claim text.