Compositions and methods for detection of beta n methylamino l alanine in cu zn superoxide dismutase 1
AssigneeNorth Carolina State University
InventorsDavid C. Muddiman, Philip L. Loziuk, Michael Bereman
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Abstract
Provided herein are isotopically labeled reagents, including isotopically labeled small molecules and peptides, that can be used to detect and/or quantify β-N-methylamino-L-alanine (BMAA) in the Cu/Zn Superoxide Dismutase 1 (SOD1) protein. Further provided are methods for detecting, preventing, or treating amyotrophic lateral sclerosis in a subject using isotopically labeled reagents to detect and/or quantify β-N-methylamino-L-alanine (BMAA) in the Cu/Zn Superoxide Dismutase 1 (50D1) protein. Further provided are isotopically labeled reagents and methods for detecting BMAA in additional proteins from patient samples.
Claims21
A polypeptide comprising a sequence that is at least 85% identical to SEQ ID NO:
- 1, wherein amino acid position 107 comprises a BMAA residue which is isotopically labeled and is defined by the formula below
- wherein the 13C isotopic enrichment factor for dC is at least 25;
- and
- wherein the 15N isotopic enrichment factor for fN is at least 100.
A polypeptide comprising a sequence that is at least 85% identical to SEQ ID NO:
- 2, wherein amino acid position 107 comprises a BMAA residue which is isotopically labeled and is defined by the formula below
- wherein the 13C isotopic enrichment factor for dC is at least 25;
- and
- wherein the 15N isotopic enrichment factor for fN is at least 100.
-111. (canceled)
Dependencies are inferred from the claim text.