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AssigneeHain Lifescience GmbH
InventorsMartin GUTEKUNST, Hans-Joachim POLLAND
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The invention relates to a method and a device for optically exciting a plurality of analytes (12) in an array of reaction vessels (14) and for sensing fluorescent light from the analytes (12), wherein excitation light from an excitation light source (38) is supplied to the analytes (12) and wherein fluorescent light from the analytes (12) is simultaneously supplied to a fluorescent detector (34), wherein a plurality of beam bundles (30) of the excitation light is directed into the reaction vessels (14) below the cover (28) through an array of holes (26) in a cover (28) arranged above the array of reaction vessels (14) and a plurality of beam bundles (32) of the fluorescent light from the reaction vessels (14) reaches the fluorescent detector (34). According to the invention it is suggested that the main beams (76) of the plurality of beam bundles (30) of the excitation light diverge within and below the holes (26) into the reaction vessels (14) and/or that the main beams (78) of the plurality of beam bundles (32) of the fluorescent light converge within and above the holes (26) toward the fluorescent detector (34).
A method for optically exciting a plurality of analytes in an array of reaction vessels and for sensing fluorescent light from the analytes, wherein excitation light from an excitation light source is supplied to the analytes in the reaction vessels and wherein the fluorescent light from the analytes in the reaction vessels is supplied to a fluorescent detector, wherein a plurality of beam bundles of the excitation light is directed into the reaction vessels below the cover through an array of holes in a cover arranged above the array of reaction vessels and at the same time a plurality of beam bundles of the fluorescent light from the reaction vessels reaches the fluorescent detector, characterized in that the plurality of beam bundles of the excitation light with diverging main beams passes through the holes from above and is directed into the reaction vessels and in that the plurality of beam bundles of the fluorescent light with converging main beams passes through the holes from below from the reaction vessels and then reaches the fluorescent detector, wherein no field lens is used.
A device for optically exciting a plurality of analytes and for sensing fluorescent light from the analytes without a field lens having an array of reaction vessels for receiving the analytes, an excitation light source for radiating the analytes in the reaction vessels with excitation light, a fluorescent detector for sensing fluorescent light from the analytes in the reaction vessels at the same time, and a cover that is arranged above the array of reaction vessels and has an array of holes through which a plurality of beam bundles of the excitation light from the excitation light source can enter the reaction vessels and a plurality of beam bundles of the fluorescent light from the analytes can reach the fluorescent detector, characterized in that the main beams of the plurality of beam bundles of the excitation light diverge within and below the holes into the reaction vessels and in that the main beams of the plurality of beam bundles of the fluorescent light converge within and above the holes toward the fluorescent detector.
Dependencies are inferred from the claim text.