Pd-1 car nk-92 cell and preparation method and use thereof
AssigneeASCLEPIUS (Suzhou) TECHNOLOGY COMPANY GROUP CO., LTD.
InventorsHuashun LI, Baolei WANG, Kunkun HAN, Baoyong REN
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Abstract
Provided are a PD-1 CAR NK-92 cell and a preparation method and use thereof. The PD-1 CAR NK-92 cell expresses PD-1-CD8™-4-1BB-CD3ζ fusion protein in NK-92 cells. The PD-1 CAR NK-92 is obtained by infecting an NK92 cell line with a PD-1 CAR molecule and obtaining monoclonal cells by means of flow screening, and culturing and expanding CAR NK92 monoclonal cell strains with stable traits and a high killing activity. The cells can be produced on a large scale, can be used in different patients without GVHR rejection, and have a specific killing activity and significant therapeutic effect on tumors.
Claims20
A PD-1 CAR NK-92 cell with expression of a PD-1-CD8-4-1BB-CD3ζ fusion protein in a NK-92 cell.
A gene encoding a fusion protein PD-1-CD8-4-1BB-CD3ζ, wherein the PD-1 protein in the PD-1-CD8-4-1BB-CD3ζ fusion protein has:
- a) an amino acid sequence as shown in SEQ ID NO:
- 5, or
- b) an amino acid sequence derived from a) by substituting and/or deleting and/or adding one or several amino acid residues and having the same function.
Dependencies are inferred from the claim text.