Dna nanorobot and methods of use thereof

A DNA nanostructure nanorobot comprising:

• a single stranded DNA scaffold strand comprising M13 phage DNA;
• a plurality of staple strands 13-204 of DNA, wherein the plurality of staple strands hybridized to the DNA scaffold forms a rectangular sheet having a top surface and a bottom surface, and four corners;
• at least six fastener strands of DNA, wherein each fastener strand of DNA is capable of fastening the rectangular sheet into a tube-shaped origami structure;
• four DNA capture strands, wherein each capture strand is operably linked to a thrombin;
• and
• at least four targeting strands, wherein each targeting strand is operably linked to an aptamer specific for nucleolin;
• a plurality of imaging strands comprising extended ssDNA sequences that hybridized to fluorescent dye-labeled ssDNA.