Delivery Compositions, and Methods of Making and Using Same
AssigneeThomas Jefferson University
InventorSunday SHOYELE
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Abstract
This invention provides compositions comprising at least one protein nanoparticle comprising a protein and a stealth polymer. In certain embodiments, the nanoparticle further comprises a therapeutic agent, such as but not limited to a miRNA and/or siRNA. In other embodiments, the nanoparticle further comprises a cell surface receptor ligand. Also included in the invention are methods of preparing the compositions of the present invention, and methods of treating, ameliorating or preventing a disease or disorder in a subject using the compositions of the present invention.
Claims32
A protein-containing nanoparticle,
- wherein the core of the nanoparticle comprises at least one protein selected from the group consisting of a plasma protein, an IgG, a cytokine, an immunomodulator, an antigen, a hormone, and an enzyme,
- wherein the at least one protein is in a neutral state in the nanoparticle,
- wherein the nanoparticle is surrounded by a layer comprising a stealth polymer,
- wherein the at least one protein is conjugated with at least one cell surface receptor ligand, wherein at least a fraction of the ligand is displayed on the outer surface of the surrounding layer of the nanoparticle.
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A method of preparing at least one stealth polymer-coated protein nanoparticle, the method comprising:
- adjusting the pH of a first solution comprising a protein to about the isoelectric point of the protein, thereby forming a first protein nanoparticle, which comprises at least a fraction of the protein;
- wherein, if the protein in the first protein nanoparticle is not conjugated to at least one cell surface receptor ligand, the protein in the first protein nanoparticle is further conjugated with the at least one cell surface receptor;
- and,
- contacting the first protein nanoparticle with a second solution comprising a stealth polymer, wherein the concentration of the stealth polymer in the second solution ranges from about 0.1% to about 20,000% of the CMC of the stealth polymer,
- thereby forming the at least one stealth polymer-coated protein nanoparticle.
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A kit comprising a stealth polymer, a protein conjugated with a cell surface receptor ligand, an applicator, and an instructional material for the use of the kit, and wherein the instruction material comprises instructions for preparing a stealth polymer-coated protein nanoparticle wherein at least a fraction of the ligand is displayed on the outer surface of the stealth polymer coating of the nanoparticle.
Dependencies are inferred from the claim text.