Nanoelectroablation control and vaccination

A device comprising:

• a processor;
• a memory communicatively coupled with the processor and storing instructions, the instructions, when executed by the processor, configure the device to:
• pass multiple treatments of sub-microsecond pulsed electric fields with different parameters through tumor cells of a first subject;
• select or allow selection of optimized parameters to formulate an effective sub-microsecond pulsed electric fields treatment which increases or maximizes apoptosis of the tumor cells of the first subject;
• and
• use the formulated sub-microsecond pulsed electric fields treatment with the optimized parameters to treat the tumor cells or other tumor cells of the first subject or a different subject with a same type of tumor cells.

A method of tumor vaccination, the method comprising

• selecting, from previous sub-microsecond pulsed electric fields treatments performed on different tumorous cells, parameters for a sub-microsecond pulsed electric fields treatment of tumor cells of a subject, wherein the parameters are selected based on an identifier of the subject or a type of the tumor cells;
• applying the sub-microsecond pulsed electric fields treatment with the selected parameters to the tumor cells of the subject;
• and
• generating a vaccine that includes apoptotic cells from the treated tumor cells.