Identification of porcine xenoantigens
AssigneeIndiana University Research and Technology Corporation
InventorBurcin Ekser
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Abstract
Provided herein are genetically modified pigs, porcine organs, tissue, and cells having a reduced propensity to cause a rejection response in a human subject following xenotransplantation. In particular, provided herein are genetically modified pigs lacking nonGal xenoantigens, and porcine cells, tissues, and organs obtained from such genetically modified pigs that are suitable for transplantation into a human. Also provided herein are methods of improving a rejection related symptom in a human subject.
Claims12
A transgenic pig whose genome comprises genetic modifications that result in one or more of a disrupted porcine CD37 (pCD37) gene and a disrupted porcine CD81 (pCD81) gene, wherein no detectable level of one or both of pCD37 and pCD81 protein is present as compared to a wild-type pig, and when tissue from said transgenic pig is transplanted into a human, a rejection related symptom is improved as compared to when tissue from a wild-type pig is transplanted into a human.
A genetically modified porcine cell, wherein the genetic modifications comprise modifications to the genome of the porcine cell that result in one or more of (i) the lack of any expression of functional pCD37 (pCD37KO) and (ii) the lack of any expression of functional pCD81 (pCD81KO), wherein said genetically modified porcine cell exhibits reduced binding to human immunoglobulins relative to a porcine cell lacking said genetic modifications.
A method of improving a rejection related symptom in a human subject comprising transplanting porcine transplant material having reduced levels of pCD37 antigens and reduced levels of pCD81 antigens into a human subject in need of a transplant, wherein a rejection related symptom is improved as compared to when porcine transplant material from a wild-type pig is transplanted into a human subject.
Dependencies are inferred from the claim text.