Means and methods for improved regeneration
AssigneesVib Vzw, Universiteit Gent
InventorsLieven De Veylder, Jefri Heyman
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Abstract
The present invention relates to the field of plant genetic engineering, particularly the modulation of gene expression in plant cells for improved regeneration competence. The invention discloses means and methods wherein the Ethylene Response Factor (ERF) and Phytochrome A Signal Transduction 1 (PAT1) transcription factor complex activity in plant cells grants increased regeneration.
Claims23
A composition comprising:
- a first chimeric gene construct and a second chimeric gene construct;
- the first chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a DNA region encoding an Ethylene Response Factor (ERF) comprising an SCL/PAT1 interaction motif, and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant, and
- the second chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a DNA region encoding Phytochrome A Signal Transduction 1 (PAT1), and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant.
A chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a multicistronic DNA region encoding an Ethylene Response Factor (ERF) comprising an SCL/PAT1 interaction motif, coupled to a DNA region encoding PAT1, and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant.
A method of increasing regeneration of plant tissue cells and/or increasing callus formation of plant tissue cells, the method comprising:
- ectopically expressing a DNA region encoding Phytochrome A Signal Transduction 1 (PAT1) in a plant, plant cell, or plant seed;
- wherein the DNA region encoding PAT1 is located in a chimeric gene construct and is operably linked to:
- a plant expressible promoter;
- and
- a 3′ end region comprising transcription termination and polyadenylation signal functioning in cells of a plant.
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Dependencies are inferred from the claim text.