Protein Reporters for Ultrasensitive Detection Methods
AssigneeThe Trustees of the University of Pennsylvania
InventorsIvan J. Dmochowski, Yanfei Wang, Benjamin W. Roose, Serge Zemerov, Zhuangyu Zhao
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions and methods using protein reporters as imaging agents in 129Xe NMR and MRI applications. It is described that bla and MBP are genetically-encoded proteins that induce a detectable chemical shift during 129Xe NMR, allowing for use as protein reporters in research and clinical applications.
Claims38
A composition comprising an imaging agent comprising one or more protein reporters selected from the group consisting of TEM-1 β-lactamase (bla), maltose-binding protein (MBP), ribose-binding protein (RBP), a fragment thereof, and a mutant thereof.
A composition comprising an isolated nucleic acid molecule comprising a nucleotide sequence encoding a protein reporter selected from the group consisting of:
- TEM-1 β-lactamase (bla), a fragment of bla, or a mutant of bla.
A composition comprising an isolated nucleic acid molecule comprising a nucleotide sequence encoding a protein reporter selected from the group consisting of maltose-binding protein (MBP), a fragment of MBP, and a mutant of MBP, ribose-binding protein (RBP), a fragment of RBP, and a mutant of RBP.
A method comprising the steps of:
- i. administering to a cell or subject one or more protein reporters comprising bla, MBP, RBP, a fragment thereof, a mutant thereof, or combination thereof;
- ii. administering hyperpolarized xenon to the cell or subject;
- iii. obtaining magnetic resonance data of a target site of the cell or subject;
- iv. analyzing the data to produce a magnetic resonance image of the target site.
A method comprising the steps of:
- i. administering to a cell or subject one or more isolated nucleic acid molecules comprising one or more nucleotide sequences encoding one or more protein reporters comprising bla, MBP, RBP, a fragment thereof, a mutant thereof, or combination thereof;
- ii. administering hyperpolarized xenon to the cell or subject;
- iii. obtaining magnetic resonance data of a target site of the cell or subject;
- iv. analyzing the data to produce a magnetic resonance image of the target site.
Dependencies are inferred from the claim text.