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AssigneeThe Regents of the University of California
InventorsJohn E. Dueber, Ryan Joseph Protzko
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The present disclosure provides methods of producing commodity products, the methods involving culturing a host cell that is genetically modified to produce a uronate dehydrogenase (UDH) that converts a sugar acid to its corresponding 1,5-aldonolactone, that uses NADP+ or NAD+ as a cofactor, and that produces NADPH or NADH, respectively, where the host cell coexpresses an endogenous or a heterologous reductase that utilizes the produced NADPH or NADH to generate the commodity product or a precursor thereof. The present disclosure provides a method of producing downstream products of glycerol and pyruvate in a genetically modified microbial host cell, the method involving culturing a genetically modified microbial host cell of the present disclosure in a culture medium comprising D-galacturonic acid. The present disclosure provides variant UDH polypeptides that utilize NADP+, nucleic acids encoding the variant UDH polypeptides; and host cells genetically modified with the nucleic acids.
A genetically modified host cell genetically modified with:
A method for producing mucic acid and a product of an anabolic pathway or a catabolic pathway that requires an active reductase that utilizes NADH or NADPH, the method comprising culturing, in a culture medium, a genetically modified host cell, wherein the genetically modified host cell is genetically modified with:
A genetically modified, fermentable host cell genetically modified with a heterologous nucleic acid comprising a nucleotide sequence encoding a heterologous uronate dehydrogenase (UDH), wherein the heterologous UDH converts a sugar acid to its corresponding 1,5-aldonolactone, and wherein heterologous UDH uses NADP+ as a cofactor, and produces NADPH.
The method of any one of claims 72-78, wherein the aldaric acid is mucic acid, and wherein the mucic acid is recovered from the cell, the culture medium, or both the cell and the culture medium.
A host cell genetically modified to convert D-galacturonic acid to a commodity product or precursor thereof, wherein the host cell is genetically modified with one or more heterologous nucleic acids comprising nucleotide sequences encoding:
A variant uronate dehydrogenase (UDH), the variant UDH comprising one or more amino acid substitutions in the NAD+ binding motif such that the variant UDH binds NADP+.
A genetically modified eukaryotic host cell, wherein the genetically modified eukaryotic host cell is genetically modified to express a heterologous D-galacturonic acid transporter.
Dependencies are inferred from the claim text.