Systems and methods for forming a nanopore in a lipid bilayer
AssigneeGenia Technologies, Inc.
InventorsRoger J.A. Chen, Randy Davis
Life of this patent
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Abstract
Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.
Claims8
A method of identifying a base of a molecule comprising:
- recording a first electrical measurement of a first portion of a molecule as the molecule moves through a nanopore under an applied electrical stimulus, wherein the first portion of the molecule includes a base;
- recording a second electrical measurement of a second portion of the molecule as the molecule moves further through the nanopore under the applied electrical stimulus, wherein the second portion of the molecule also includes the base, and wherein the second portion of the molecule is different from the first portion of the molecule;
- and
- to identifying the base of the molecule by processing the first electrical measurement and the second electrical measurement together.
A system for identifying a base of a molecule, comprising:
- a sensing circuit coupled to a nanopore;
- and
- a processor coupled to the sensing circuit, wherein the processor is programmed to:
- control the sensing circuit to record a first electrical measurement of a first portion of a molecule as the molecule moves through a nanopore under an applied electrical stimulus, wherein the first portion of the molecule includes a base;
- control the sensing circuit to record a second electrical measurement of a second portion of the molecule as the molecule moves further through the nanopore under the applied electrical stimulus, wherein the second portion of the molecule also includes the base, and wherein the second portion of the molecule is different from the first portion of the molecule;
- and
- identify the base of the molecule by processing the first electrical measurement and the second electrical measurement together.
Dependencies are inferred from the claim text.