Recombinant mumps virus vaccine
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsBiao He, Steven A. Rubin
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Abstract
The present invention provides the complete genomic sequence of the epidemic mumps virus (MuV) strain MuVIowa/US/06. Further, a reverse genetics system was constructed and used to rescue recombinant viral constructs that are attenuated compared to rMuVIowa/US/06 and JL vaccine viruses. Such constructs include viral constructs lacking the open reading frame (ORF) of the SH gene (rMuVΔSH) and/or incapable of expressing the V protein (rMuVΔV).
Claims20
An isolated nucleotide sequence comprising the cDNA sequence encoding the full length RNA genome of a mumps virus, wherein the isolated nucleotide sequence encodes a mumps virus unable to express a small hydrophobic (SH) protein product and/or express a V protein product.
An isolated nucleotide sequence comprising the cDNA sequence encoding the full length RNA genome of a mumps virus, wherein the cDNA sequence encoding the full length RNA genome of the mumps virus comprises one or more mutations to the V/I/P gene abrogating expression of the V protein.
An isolated nucleotide sequence comprising the cDNA sequence encoding a full length RNA genome of an infectious mumps virus that grows to a titer in Vero cells similar to the titer of wild type virus in Vero cells, wherein the cDNA sequence encoding the full length RNA genome of the mumps virus comprises one or more mutations to the V/I/P gene abrogating expression of the V protein.
Dependencies are inferred from the claim text.