Microbial strains and their use in animals
AssigneeDupont Nutrition Biosciences Aps
InventorsStephane Frouel, Christopher Kromm
Life of this patent
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Abstract
Bacillus and Lactobacillus strains and methods that are useful for improving the performance of aquatic animals. The invention also discloses Bacillus and Lactobacillus strains and methods that are useful for inhibiting or slowing the growth of a pathogenic agent in an aquatic animal.
Claims31
A composition comprising one or more isolated strains selected from the group consisting of Bacillus pumilis 3064, Bacillus subtilis BS 2084 (NRRL B-50013), Bacillus subtilis BS15 Ap4 (ATCC PTA-6507), Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus pumilis 119 (NRRL B-50796), Bacillus subtilis 3A-P4 (ATCC PTA-6506), Bacillus subtilis 22C-P1 (ATCC PTA-6508), Bacillus licheniformis 842 (NRRL B-50516), Bacillus subtilis BS27 (NRRL B-50105), Bacillus licheniformis BL21 (NRRL B-50134), Bacillus pumilus AGTP BS 1068 (NRRL B-50543), and Bacillus subtilis AGTP BS1069 (NRRL B-50544), Lactobacillus farcimins CNCM-I-3699, and Lactobacillus rhamnosus CNCM-I-3698, and strains having all the characteristics thereof, any derivative or variant thereof, and mixtures thereof.
A method comprising administering to an animal an effective amount of a composition comprising one or more isolated strains selected from the group consisting of Bacillus pumilis 3064, Bacillus subtilis BS 2084 (NRRL B-50013), Bacillus subtilis BS15 Ap4 (ATCC PTA-6507), Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus pumilis 119 (NRRL B-50796), Bacillus subtilis 3A-P4 (ATCC PTA-6506), Bacillus subtilis 22C-P1 (ATCC PTA-6508), Bacillus licheniformis 842 (NRRL B-50516), Bacillus subtilis BS27 (NRRL B-50105), Bacillus licheniformis BL21 (NRRL B-50134), Bacillus pumilus AGTP BS 1068 (NRRL B-50543), and Bacillus subtilis AGTP BS1069 (NRRL B-50544), Lactobacillus farcimins CNCM-I-3699, and Lactobacillus rhamnosus CNCM-I-3698, and strains having all the characteristics thereof, any derivative or variant thereof, and mixtures thereof.
An animal feed or feed additive composition, comprising one or more isolated strains selected from the group consisting of Bacillus pumilis 3064, Bacillus subtilis BS 2084 (NRRL B-50013), Bacillus subtilis BS15 Ap4 (ATCC PTA-6507), Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus pumilis 119 (NRRL B-50796), Bacillus subtilis 3A-P4 (ATCC PTA-6506), Bacillus subtilis 22C-P1 (ATCC PTA-6508), Bacillus licheniformis 842 (NRRL B-50516), Bacillus subtilis BS27 (NRRL B-50105), Bacillus licheniformis BL21 (NRRL B-50134), Bacillus pumilus AGTP BS 1068 (NRRL B-50543), and Bacillus subtilis AGTP BS1069 (NRRL B-50544), Lactobacillus farcimins CNCM-I-3699, and Lactobacillus rhamnosus CNCM-I-3698, and strains having all the characteristics thereof, any derivative or variant thereof, and mixtures thereof.
A method of producing one or more isolated strains selected from the group consisting of Bacillus pumilis 3064, Bacillus subtilis BS 2084 (NRRL B-50013), Bacillus subtilis BS15 Ap4 (ATCC PTA-6507), Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus pumilis 119 (NRRL B-50796), Bacillus subtilis 3A-P4 (ATCC PTA-6506), Bacillus subtilis 22C-P1 (ATCC PTA-6508), Bacillus licheniformis 842 (NRRL B-50516), Bacillus subtilis BS27 (NRRL B-50105), Bacillus licheniformis BL21 (NRRL B-50134), Bacillus pumilus AGTP BS 1068 (NRRL B-50543), and Bacillus subtilis AGTP BS1069 (NRRL B-50544), Lactobacillus farcimins CNCM-I-3699, and Lactobacillus rhamnosus CNCM-I-3698, and strains having all the characteristics thereof, any derivative or variant thereof, and mixtures thereof, comprising:
- (a) growing, in a liquid broth, a culture including the one or more strain(s);
- and (b) separating the one or more strains from the liquid broth.
Dependencies are inferred from the claim text.