Identification and monitoring of cleaved immunoglobulins by molecular mass
AssigneeMayo Foundation for Medical Education and Research
InventorsDavid R. Barnidge, David L. Murray
Life of this patent
Estimating the term…
Abstract
This document relates to materials and methods for identifying and monitoring immunoglobulin cleavage (e.g., IgG cleavage) in a sample, such as a biological sample, using mass spectrometry techniques.
Claims53
A method for detecting IgG cleavage in a patient, the method comprising:
- a. providing a sample comprising IgG from the patient;
- b. subjecting the sample to a mass spectrometry technique to obtain a mass spectrum of the sample;
- and
- c. identifying the presence of an IgG cleavage fragment.
A method for identifying plasmin activation in a patient, the method comprising:
- a. providing a sample comprising IgG from the patient;
- b. subjecting the sample to a mass spectrometry technique to obtain a mass spectrum of the sample;
- and
- c. identifying the presence of an IgG cleavage fragment.
A method for detecting complement activation in a patient, the method comprising:
- a. providing a sample comprising IgG from the patient;
- b. subjecting the sample to a mass spectrometry technique to obtain a mass spectrum of the sample;
- and
- c. identifying the presence of an IgG cleavage fragment.
A method for identifying an inflammatory condition in a patient, the method comprising:
- a. providing a sample from the patient;
- b. subjecting the sample to a mass spectrometry technique to obtain a mass spectrum of the sample;
- and
- c. identifying the presence of an IgG cleavage fragment.
A method for monitoring plasmin activation in a subject comprising:
- a. providing a first sample of the subject;
- b. providing a second sample of the subject;
- c. subjecting the first and second samples to a mass spectrometry technique to obtain a mass spectrum of the first and second samples;
- d. determining the amount of IgG cleavage fragment in the first and second samples;
- and
- e. comparing the amount of the IgG cleavage fragment in the first and second samples.
A method for monitoring a treatment of an immune disease in a patient comprising:
- a. providing a first sample of the patient obtained before the treatment;
- b. providing a second sample of the patient obtained during or after the treatment;
- c. subjecting the first and second samples to a mass spectrometry technique to obtain a mass spectrum of the first and second samples;
- d. determining the amount of IgG cleavage fragment in the first and second samples;
- and
- e. comparing the amount of the IgG cleavage fragment in the first and second samples.
Dependencies are inferred from the claim text.