Method and device for analyzing protein or peptide
AssigneeShimadzu Corporation
InventorsSadanori SEKIYA, Hidenori TAKAHASHI
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AssigneeShimadzu Corporation
InventorsSadanori SEKIYA, Hidenori TAKAHASHI
Estimating the term…
In a mass spectrum of fragment ions obtained by dissociating peptide-derived ions using the technique of irradiating the ions with hydrogen radicals, either pairs of a-type and c-type ions or those of z-type and z-type ions are characteristically observed. Since the mass difference of those ion pairs is previously known, a pair peak searcher 92 searches for pair peaks having a predetermined mass difference in a mass spectrum created by a mass spectrum creator 91, and adds to the detected pair peaks a piece of information indicating that they are pairs of a-type and c-type ions or those of x-type and z-type ions. When estimating the amino acid sequence of the peptide by a database search, a protein identifier 93 uses the ion-pair information in addition to the m/z value of each peak, whereby the accuracy of the estimation or identification the amino acid sequence can be improved.
An analyzing method for identifying a protein or peptide, or for determining an amino acid sequence of a protein or peptide, the method comprising:
An analyzing device for identifying a protein or peptide, or for determining an amino acid sequence of a protein or peptide, the device comprising:
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