Arrays, substrates, devices, methods and systems for detecting target molecules
AssigneeCalifornia Institute of Technology
InventorsRong Fan, Habib Ahmad, James R. Heath
Life of this patent
Estimating the term…
Abstract
Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.
Claims24
An array for detecting at least one target in a sample, the array comprising:
- at least one capture agent or component thereof attached to a substrate, the at least one capture agent capable of specifically binding the at least one target to form a capture agent target binding complex,
- the at least one capture agent or component thereof arranged on the array so that capture agent target binding complexes are detectable along substantially parallel lines forming a barcoded pattern.
3-5. (canceled)
7-8. (canceled)
12-13. (canceled)
16-19. (canceled)
A substrate for detecting at least one detectable target, the substrate configured to allow attachment of said at least one detectable target on the substrate along substantially parallel lines, the substantially parallel lines forming a barcoded pattern.
A method to attach a molecule on a fluidic support along a predetermined microfluidic pattern, the method comprising:
- providing a mold, the mold comprising fluidic channels, each microfluidic channel having an inlet and an outlet, each of the outlets of the channels configured to form part of the predetermined pattern,
- providing the support, said support suitable to be coupled with the mold,
- coupling the mold with the support,
- providing the molecule in the fluidic channels for a time and under conditions to allow attachment of the molecule on the support;
- and
- decoupling the mold from the support.
27-28. (canceled)
A system to attach a molecule on a fluidic support along a predetermined microfluidic pattern, the system comprising:
- a mold, the mold comprising microfluidic channels, the microfluidic channels having an inlet and an outlet, the outlets of the channels configured to form part of the predetermined pattern, and
- a support suitable to be coupled with the mold.
32-33. (canceled)
Dependencies are inferred from the claim text.