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US 2019/0195866 A1Published application
Improved pharmacokinetic assays for immunoglobulin single variable domains
AssigneeAblynx N.V.
InventorsVeerle Snoeck, Lieselot Bontinck, Sofie Poelmans, Kjell Mortier, Marie-Ange Buyse, Lies Dekeyzer, Judith Baumeister
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Abstract
The present invention generally relates to improved pharmacokinetic assays for measuring levels of immunoglobulin single variable domains (also referred to herein as “ISVs” or “ISVDs”) and of proteins and polypeptides that comprise at least one ISV (as further described herein) in biological samples.
Claims8
Method for determining the amount and/or concentration in a sample of at least one ISVD, or of a protein or polypeptide that comprises at least one ISVD, which method comprises the steps of;
- a) contacting the sample with a capturing agent, such that the ISVD, protein or polypeptide is captured by said capturing agent;
- b) contacting the ISVD, protein or polypeptide captured by the capturing agent with a detection agent, such that said detection agent binds to the ISVD, protein or polypeptide captured by the capturing agent;
- c) generating a signal corresponding to the amount of detection agent bound to the ISVD, protein or polypeptide captured by the capturing agent,
- in which said method is performed in the presence of a quencher, said quencher being a protein or polypeptide to which pre-existing antibodies can bind but to which neither the capturing agent nor the detection agent can bind.
1 more element
Dependencies are inferred from the claim text.