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US 2019/0195855 A1Published application
Vitamin b2 detection by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsChangming Yang, Sum Chan
Life of this patent
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Abstract
Methods are described for measuring the amount of a vitamin B2 in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying vitamin B2 in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric techniques.
Claims20
A method for determining the amount of vitamin B2 in a biological sample from a human, said method comprising:
- (a) adding an internal standard to the sample;
- (b) subjecting the sample to high performance liquid chromatography (HPLC);
- (c) ionizing vitamin B2 and the internal standard under conditions suitable to produce one or more ions detectable by tandem mass spectrometry;
- (d) determining the amount of said one or more ions by tandem mass spectrometry;
- and
- (e) comparing the amount of said one or more ions of vitamin B2 and said one or more ions of the internal standard to determine the amount of vitamin B2 in the sample.
3 more elements
A method for determining the amount of vitamin B2 in a biological sample from a human, said method comprising:
- (a) adding an internal standard to the sample;
- (b) precipitating protein from the sample;
- (c) subjecting the sample to high performance liquid chromatography (HPLC);
- (c) ionizing vitamin B2 and the internal standard under conditions suitable to produce one or more ions detectable by tandem mass spectrometry;
- (d) determining the amount of said one or more ions by tandem mass spectrometry;
- and
- (e) comparing the amount of said one or more ions of vitamin B2 and said one or more ions of the internal standard to determine the amount of vitamin B2 in the sample.
4 more elements
Dependencies are inferred from the claim text.