Systems, methods and devices addressing sample extraction and injection problems in chromatography

A method of preparing a sample feed in a chromatography solution comprising:

• pressurizing a first flowstream through a valve to a chromatography column with a mixture, the mixture comprising mobile phase and co-solvent;
• pressurizing a second flowstream by introducing the mixture into an extraction vessel, the extraction vessel containing a sample, and then introducing the mixture with sample through the valve and to a sample loop;
• and
• actuating the valve to introduce the mixture with sample in the sample loop into the chromatography column.

A method for obtaining a substantially equal concentration of sample throughout a feed solution for a chromatography column, the feed solution comprising a sample, a mobile phase, and a co-solvent, by

• pressurizing a flowstream to a chromatography column with a mixture, the mixture comprising the mobile phase and the co-solvent;
• preparing the feed solution in a primary extraction-pressurization system by supplying the mixture to an extraction vessel in the primary extraction-pressurization system and subsequently supplying the mixture supplied with sample to a reservoir in the primary extraction-pressurization system to create a feed solution from the mixture supplied with sample;
• and
• supplying the feed solution from the primary extraction-pressurization system to the flowstream.