Measuring device with injector and spray deflector

Method for measuring a light signal of a sample in a well (9) of a microplate (8), characterized by the following steps:

• providing a measuring device (1), which comprises:
• a first injector (2) for dispensing a reagent into a well (9) of a microplate (8), wherein the injector (2) comprises an injector needle (6) having an outlet end (25),
• an optical device (10,10′,10″) having an optical axis (20,31,32) extending substantially perpendicularly to the microplate (8) and a measuring region (23) surrounding the optical axis (20,31,32) for measuring a light signal of a sample in a well (9) of the microplate (8),
• wherein the injector needle (6) of the injector (2) extends below the optical device (10,10′,10″) and opens with its outlet end (25) into the measuring region (23) of the optical device (10,10′,10″), and
• a spray deflector (16), which is arranged above the outlet end (25) of the injector (2) and below the optical device (10,10′,10″), and
• which extends substantially perpendicularly to the optical axis (20) and completely covers at least the measuring region (23) of the optical device (10,10′,10″),
• dispensing a reagent into a well (9) of the microplate (8) by means of the injector (2),
• measuring a light signal of the sample in a well (9) of the microplate (8) along the optical axis (20,31,32) and in the measuring region (23) of the optical device (10,10′,10″),
• wherein the dispensing of the reagent and the measurement of the light signal of the sample are carried out in the same well (9).

Method for measuring a light signal of a sample in a well (9) of a microplate (8), characterized by the following steps:

• providing a measuring device (1), which comprises:
• a first injector (2) for dispensing a reagent into a well (9) of a microplate (8), wherein the injector (2) comprises an injector needle (6) having an outlet end (25),
• an optical device (10,10′,10″) having an optical axis (31) extending substantially perpendicularly to the microplate (8) and a measuring region (23) surrounding the optical axis (31) for measuring a light signal of a sample in a well (8) of the microplate (8),
• wherein the injector needle (6) of the first injector (2) extends to below the optical device (10,10′,10″) and opens with its outlet end (25) into the measuring region (23) of the optical device (10,10′,10″),
• a spray deflector (16), which is arranged above the outlet end (25) of the injector (2) and below the optical device (10,10′,10″), and which extends substantially perpendicularly to the optical axis (31) and completely covers at least the measuring region (23) of the optical device (10,10′,10″), and
• a second injector (3) comprising an injector needle (7) and an outlet end (26), wherein the outlet end (26) of the second injector (3) is spaced from the outlet end of the first injector (2) by a distance from the outlet end (25) of the first injector (2) corresponding to one or a multiple of an axial distance of the wells of the microplate (8),
• dispensing a first reagent into a well (9) of the microplate with the second injector (3),
• dispensing a second reagent with the first injector (2) into a well (9),
• measuring a light signal of the sample in a well (9) of the microplate (8) along the optical axis (20) and in the measuring region (23) of the optical device (10,10′,10″),
• wherein the dispensing of the second reagent and the measurement of the light signal of the sample are carried out in the same well (9).