Method and device for chemiluminescence-based analysis
AssigneeB.G. Negev Technologies and Applications Ltd., At Ben-Gurion University
InventorAlina KARABCHEVSKY
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Abstract
A method for detecting an analyte reactive towards luminol, comprising the steps of: feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel; detecting the light emitted due to a chemiluminescence reaction taking place in said channel; and discharging a reaction mass from said channel, characterized in that the average diameter of the metal nanoparticles is greater than 25 nm. Also provided is a microfluidic device for carrying out the method.
Claims12
A method for detecting an analyte reactive towards luminol, comprising the steps of:
- feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel;
- detecting the light emitted due to a chemiluminescence reaction taking place in said channel;
- and
- discharging a reaction mass from said channel,
- wherein the average diameter of the metal nanoparticles is greater than 25 nm.
A method for detecting an analyte reactive towards luminol, comprising the steps of:
- feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel;
- detecting the light emitted due to a chemiluminescence reaction taking place in said channel;
- and
- discharging a reaction mass from said channel,
- wherein the channel consists of two straight, parallel, sections connected by a curved section which joins the straight sections smoothly, wherein the length L of each of the individual straight sections is from 400 μm to 1000 μm and the radius R of the curved section is from 50 μm to 200 μm.
10-12. (canceled)
A microfluidic device adapted for luminescence-based detection, comprising:
- A) a curved channel having cross-sectional dimension from 0.15 mm to 0.5 mm;
- wherein the flow channel consists of a pair of straight, essentially parallel sections connected by a curved section joining the straight sections smoothly, wherein the length L of each of the individual straight sections is from 400 μm to 1000 μm and the radius R of the curved section is from 50 μm to 200 μm;
- B) a plurality of reservoirs and pumps for holding and delivering into said flow channel:
- a solution of a luminescence reagent,
- a luminescence enhancer comprising noble metal nanoparticles;
- a sample comprising an analyte reactive towards luminol;
- wherein said reservoirs are connected through tubes to input opening(s) of the flow channel,
- C) a detector for measuring the intensity of the light emitted by the luminescence reaction;
- and optionally
- D) a vessel to which the reaction mixture is withdrawn.
Dependencies are inferred from the claim text.