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US 2019/0195774 A1Published application
High accuracy 5-part differential with digital holographic microscopy and untouched leukocytes from peripheral blood
AssigneeSiemens Healthcare GmbH
InventorsNoha Youssry El-Zehiry, Oliver Hayden, Ali Kamen, Lukas Richter, Manfred Stanzel, Matthias Ugele, Daniela Seidel, Gaby Marquardt, Oliver Schmidt
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Abstract
The present invention relates to an improved method for marker-free detection of a cell type of at least one cell in a medium using microfluidics and digital holographic microscopy, as well as a device, particular for carrying out the method.
Claims15
A method for marker-free detection of a cell type of at least one cell in a medium, comprising:
- flowing a medium comprising at least one cell into a microfluidic device,
- obtaining an image of the at least one cell in the microfluidic device by a digital holographic microscopic device, wherein the image is obtained with a depth of field of less than 6 μm, and
- determining the cell type of the at least one cell.
A device for marker-free detection of a cell type of at least one cell in a medium, comprising:
- a digital holographic microscopic device with a depth of field of less than 6 μm;
- a microfluidic device;
- and
- a detection system configured to determine the cell type of the at least one cell.
1 more element
Dependencies are inferred from the claim text.