Calibration of a chip-based microfluidic calorimeter

Abstract

The invention provides a calibration method for calibrating a chip-based microfluidic calorimeter, wherein the chip-based microfluidic calorimeter comprises one or more thermopiles, wherein the calibration method uses the deprotonating reaction of a phosphate group, the method comprising: providing calibration liquids comprising (i) a buffer with a pH range of at least 7-9 and (ii) a first compound with a phosphate group which is protonated in a pH range of at least 3-6, and mixing these calibration liquids in the chip-based microfluidic calorimeter to provide a calibration liquid mixture whereby heat is generated, measuring the heat by the thermopiles and thereby providing a corresponding thermopile signal, and calibrating the chip-based microfluidic calorimeter by relating the thermopile signal to reference data of the deprotonating reaction.

Dependencies are inferred from the claim text.