Determination of microorganism operational taxonomic unit and sequence-assisted separation

An electronic device, comprising at least one processor;

• and a memory communicably connected with the at least one processor for storing instructions executable by the at least one processor, execution of the instructions by the at least one processor causes the at least one processor to:
• obtain a relative abundance value of each of qualified sequences of a phylogenetically informative gene in microorganisms contained in the sample, wherein the total relative abundance of all qualified sequences is 100%;
• rank from high to low all qualified sequences by their respective relative abundance value, and separating the qualified sequences into a high abundance group and a low abundance group, wherein the high abundance group consists of qualified sequences whose abundance values are higher than the those in the low abundance group and collectively account for about 70%-80% of the total abundance;
• and the low abundance group consists of the remaining qualified sequences which account for about 20%-30% of the total abundance;
• delineate OTUs in the sample using only qualified sequences in the high abundance group to obtain Tentative OTUs;
• and
• re-map qualified sequences in the low abundance group to the Tentative OTUs, and assigning them individually to a suitable Tentative OTUs only if they the qualified sequence has at least 90% sequence similarly to the OTU Sequence, to arrive at the final definition of OTUs.

A non-transitory computer-readable storage medium storing executable instructions that, when executed by an electronic device, cause the electronic device to:

• obtain a relative abundance value of each of qualified sequences of a phylogenetically informative gene in microorganisms contained in the sample, wherein the total relative abundance of all qualified sequences is 100%;
• rank from high to low all qualified sequences by their respective relative abundance value, and separating the qualified sequences into a high abundance group and a low abundance group, wherein the high abundance group consists of qualified sequences whose abundance values are higher than the those in the low abundance group and collectively account for about 70%-80% of the total abundance;
• and the low abundance group consists of the remaining qualified sequences which account for about 20%-30% of the total abundance;
• delineate OTUs in the sample using only qualified sequences in the high abundance group to obtain Tentative OTUs;
• and
• re-map qualified sequences in the low abundance group to the Tentative OTUs, and assigning them individually to a suitable Tentative OTUs only if they the qualified sequence has at least 90% sequence similarly to the OTU Sequence, to arrive at the final definition of OTUs.